this is a match C DNA
Cudoniella "sp-PA01"
Novel to all DNA repositories
DNA - ITS - Nanopore
voucher number now A-3049 dont know what happened
collectors V
https://www.inaturalist.org/observations/187333041

Tiny, under 0.5mm apothecia on decorticated hardwood stick, likely from Acer.
Ascospores measure in H2O
(8.7) 9.1 - 11.5 (12.6) × (2.5) 2.6 - 3 µm
Q = (3.2) 3.3 - 4 (4.5) ; N = 16
Me = 10.2 × 2.8 µm ; Qe = 3.6

Asci IKI+bb, croziers(-), measure in H2O
(44.2) 44.21 - 51.19 (51.2) × (6.6) 6.64 - 7.69 (7.7) µm
Q = (5.8) 5.82 - 7.7 ; N = 4
Me = 46.6 × 7.3 µm ; Qe = 6.4

Paraphyses cylindrical, septated, about 2um width, no VBs.

Tiny white substipitate discomycete on leaves. Apothecia with long marginal hairs around 100x3um with many septa.
Asci 8-spored, croziers(+), IKI-, 37-46x5.9-6.5um.
Ascospores are subfusiform to cylindrical, eguttulate, overmatured with one septum.
Paraphyses are filiform without VBs.

obtained in moist chamber from extremely rotten, wet wood of an old huge, long dead, Acer saccharum, sample was collected from this location on the 27 July 2022

Conidiomata localized, well defined sporodochia to very poorly defined, short stout synnemata, about 0.5mm tall at most, virtually identical to conidiomata produced by Hyalotrochophora lignatilis. Hypha. sinuous, thin, 0.6 - 0.8µm, appear to be sparsely branched and with few septa. Conidiophore probably undifferentiated from hyphae, conidiogenesis not observed but probably apical.

Conidia hyaline, coiled 1 1⁄2 time, with (5)7-8(9) septa, conidia are 12 - 15µm wide and individual filament are 3.5 - 5µm wide. A thin mucilaginous sheet appear to cover the conidia.

This species appear to be macroscopically very similar to Hyalotrochophora lignatilis, but easily differ by having conidia coiled 1 1⁄2 and not 1⁄2 (u shaped), with 5-9 septa (3 in H. lignatilis). Because Hyalotrochophora is monotypic, I had to look at possible option in other genera.

The species that I have found to be the most similar to this collection is Everhartia candida, described by Linder in is 1929 monograph of helicosporous hyphomycetous fungi. the protologue and illustration show a species that is extremely similar to this collection but with smaller conidia (9-12µ, filaments 3.3-5µ), the description also mention up to 7 septa in conidia (8 in the key), while several conidia in this collection had 8 septa and a few even 8 septa

The placement as Everhartia is only tentative, as E. hymenuloides, the type species might be an algae. Interestingly, the conidia of H. lignatilis also have a sheet and septa very similar to this species (see Flickr link below), and one have to wonder if the are related, especially considering the similar macromorphology, however more studies would be needed to verify this. Unfortunately, all my attempt to isolate this collection in pure culture failed, and thus for now I prefer to use the name Everhartia sp. for this collection

https://www.flickr.com/photos/31366379@N05/28569012783

@Keith_Seifert

A-3049
Collector: Cara Coulter

Spore Sizes:

(8.5)8.5-12.5 x (3.5)2.86-4.32 µm

38 spores measures from mature fresh specimen.

Specimen:
3.5cm to 2cm in length
Cap Size: .4-.6cm

Coloration:
Cream colored cap with gradual transition about halfway down the stipe to a darkened brown foot

Specimen found growing on log that was submerged in water. Half of the specimen's stipe was under water.

Habitat: Inundated sphagnum area with a mix of Hemlock, Birch, Maple and Rhododendron

On cedar small cup fungi. Apothecia white, 0.3-0.6mm in diameter with a short stipe.
Marginal hairs with crooks.
Asci 8-spored, IKI+bb Calycina type.
Ascospores hyaline, pyriform, measured
*(6.2) 6.5 - 8.1 (9.2) × (1.8) 2 - 2.4 (2.5) µm
Q = (2.9) 3 - 3.7 (3.9) ; N = 15
Me = 7.4 × 2.2 µm ; Qe = 3.3

With two hyphomycete on the same leaf.

Growing on a hardwood stick on the ground.
Tiny, apothecia about 1mm.
Asci 45-54um, IKI+b.
Paraphyses without vacuoles.
Spores smooth, hyaline, not septate, eguttulate, measure
(5.9) 6.5 - 8.7 (9.8) × (1.5) 1.6 - 2.1 (2.2) µm
Q = (3.4) 3.6 - 4.6 (5.4) ; N = 38
Me = 7.6 × 1.9 µm ; Qe = 4.1

White Lachnum sp. on plant litter and wood sticks up to an inch in diameter in a wet area.
-Hairs without oxalate crystals;
-Paraphyses without vacuoles, but very often with just one gluttule, 4.5-6um width, often wider than asci.
-Asci with croziers, 4-5 um width.
Ascospores hyaline, cylindrical, measure in H2O
(8) 8.3 - 9.9 (10.2) × (1.9) 2 - 2.3 (2.6) µm
Q = (3.6) 3.8 - 4.8 (5.5) ; N = 33
Me = 9.1 × 2.1 µm ; Qe = 4.3

On plant debris on the ground, under leaves. (Analyzed from leaves).
Apothecia 0.2-0.25mm.
Asci 8-spored with croziers, 38-46x4.4-5.6um
Paraphyses lanceolate, without vacuoles.
Marginal hairs 86-94x4.6-5um, septated with warts.

Found in my personal garden
Growing on dead, woody twigs of Malva assurgentiflora
Minute cups with a textured, greyish hymenophore, outer excipulum covered in dense white hairs. Many of the sporocarps have internal growth giving them a donut-like appearance

On herbaceous stems tiny yellows cup fungi.
Asci 8-spored, biseriate, IKI+r, croziers(+), 58x6um, 56x6um.
Ascospores subfusiform, aseptate, with few very small guttules, measured
*(9.1) 10.9 - 16.2 (17) × (1.7) 1.8 - 2.2 (2.5) µm
Q = (4.2) 4.9 - 7.9 (9.8) ; N = 23
Me = 12.9 × 2 µm ; Qe = 6.4
Paraphyses lanceolate, with 2-3 septa, with or without guttules.
Marginal hairs yellow-brown, cylindrical, septate with brown oil drops.

If interested in sphagnum bogs you can find many things simply by rinsing some sphagnum and checking the rinsate under the scope. I also like to use a moist chamber for this. All sorts of interesting things can be found in a tuft/mat of sphagnum. Rotifers, tartigrades and numerous fungal spores. Leave a tuft of moss in a moist chamber with a small amount of water at the bottom and examine the water over time. These Helicoon spores are quite distinctive. Helicoon pleuriseptatum Beverwijk from Sphagnum washes Oct and Nov 2021 Lake Verde bog and Black Ck (small patch of Sphagnum near slow rivulet).
Conidia 40-60µm diax20-30µm height, flattened, 6-10 coils/spirals, dark brown.
Mycoportal includes 22 specimens from the UK, US, NZ and Russia (Siberia). Most from bog habitats.

Collected during the 11th International Mycological Congress in San Juan, Puerto Rico. #imc11

—

Image #1:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/16, ISO 500
Color Corrected w/ X-Rite ColorChecker Passport
Image #2:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/16, ISO 200
Color Corrected w/ X-Rite ColorChecker Passport
Image #3:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/10, ISO 200
Color Corrected w/ X-Rite ColorChecker Passport
Image #4: Samsung Galaxy Note 4 + Dissecting Scope
Image #5:
100x – 3% KOH
Olympus CX30
Image #6:
400x – 3% KOH
Olympus CX30
Image #7:
1000x – 3% KOH
Olympus CX30
Image #8:
1000x – 3% KOH
Olympus CX30
Image #9:
1000x – 3% KOH
Olympus CX30
Image #10:
1000x – 3% KOH
Olympus CX30
Image #11:
1000x – 3% KOH
Olympus CX30
Image #12: habitat and host

—

—MO custom fields—

Comments: see this species list for more observations of this fungus (or group of fungi), whose identity has evaded me for upwards of six years. in each example, the yellow stipe tissue turns deep purple in the presence of KOH. excited to not only to have a collection for sequencing and culturing, but for have received the provisional ID of Lindquistia — a xylariaceous anamorph — courtesy of IMA president, Dr. Keith Seifert, who I showed this to at the recent IMC in Puerto Rico. Dr. Seifert also possesses a portion of the collection, and I am working on gathering together more material from around the world.

Originally posted to Mushroom Observer on Jul. 27, 2018.

Permis de recherche scientifique Parc National d'Oka ;

PN0 2023-005

CG3045.
Érablière à caryer, chênes, hêtres. Au sol, grégaire.
Chapeau jusqu’à 4 cm. diam, viscidule.
Lames pourprées sur les jeunes spécimens, interveinées, sinuées.
Les lames pâlissent à maturité mais garde la couleur pourprée à l'arête.
Pied jaunâtre, strié, creux, longueur jusqu’à 6 cm.
Sporée blanche.
Récolté et déterminé par Yves Lamoureux.
Collectionné par Chantal Gauthier.

Non-stromatic, unitunicate, black pyrenomycetes fungi.
Small, hard perithecia up to 0.5 mm in diameter immersed in decorticated wood, with a well-developed neck. In a mixed forest.
Mature spores hyaline, with 3 septa, measure in H2O 25-30x5.9-6.4um

On deer dung tiny orange cup fungi with hyaline marginal hairs.
Asci 8-spored.
Ascospores ellipsoid, eguttulate, measured
*(18.7) 18.73 - 20.2 (20.4) × 9.3 - 10.3 (10.9) µm
Q = (1.8) 1.9 - 2 (2.1) ; N = 8
Me = 19.4 × 9.9 µm ; Qe = 2
Marginal hairs thick-walled, aseptate.

Growing abundantly on at least 3 well-rotted fallen hardwoods in close proximity. On the sides of the logs near where they meet the forest floor but not on the very bottoms. Salix nigra, Acer sacchariferum, Fagus grandifolia, Carya sp. and Carpinus caroliniana nearby as well as possibly some other hardwood species. Odor indistinct. Attached to wispy white rhizomorphs. Stems lateral. Caps finely fuzzy. Gills starting out pure white.

Small discomycete on decorticated hardwood next to Hormomyces aurantiacus. Apothecia up to 350um sessile.
8-spored asci with croziers, not sure about IKI, could be very weak blue, but strong blue after KOH. Asci measure in H2O
(43.2) 44.4 - 52.9 (54.4) × (6.7) 6.9 - 8.3 (8.6) µm
Q = (6.1) 6.13 - 6.5 (6.6) ; N = 6
Me = 48.7 × 7.7 µm ; Qe = 6.3
Ascospores ellipsoid, hyaline OCI=1, measure in H2O in asci
(7.2) 7.4 - 8.1 (8.3) × (2.8) 3.3 - 3.4 (3.8) µm
Q = (2) 2.1 - 2.4 (2.7) ; N = 6
Me = 7.7 × 3.3 µm ; Qe = 2.3
Paraphyses are cylindrical without VBs.
Marginal hairs smooth, slightly pigmented about 50-60x2.9-4.6um.
KOH negative.

Small cupulate fungi on Populus log. Apothecia are 0.4-0.7mm in diameter, sessile. Next to Scutellinia setosa.
Asci IKI+red, croziers(+).
Ascospores were still mostly in asci, hyaline, eguttulate, subfusiform, OCI=0-1, measure
(6.9) 7 - 9.3 (9.9) × (1.9) 2 - 2.5 (2.7) µm
Q = (2.6) 2.8 - 4.4 (4.6) ; N = 18
Me = 8.3 × 2.2 µm ; Qe = 3.7
Paraphyses are slightly lanceolate, without VBs.
Marginal hairs hyaline with many septa, 122x3.7um

I haven't seen this species before.

MRD0017

Station: Forêt d'Enseignement et de Recherche du Lac Duparquet

Lieu: Rapide-Danseur, Qc

Habitat : Forêt mixte (Sapin baumier et Peuplier Faux-tremble

Substrat : sous un tronc mort

Collectionneur : Mathias Rocheleau-Duplain

Déterminateur : Mathias Rocheleau-Duplain, Jonathan Mack et Jonathan Jensen-Lynch

MRD0040

1 janvier 2024
Pont-Rouge, Québec

Habitat : Corde de bois
Substrat : Buche d'Acer sp. (Érable)

Collectionneur : Mathias Rocheleau-Duplain

Taille du champignon : moins de 1 mm.

Mode de croissance : en colonie.

San Vicente Redwoods- Mixed hardwood/conifer forest that burned in the 2020 CZU fire
Growing on dead Quercus agrifolia leaves in an area with low severity burn, Quercus agrifolia, Arbutus menziesii and Sequoia sempervirens dominant
Sporocarops about the size of mustard seed, covered in white strongly filaments. Some sporocarps have pseudo-lamellae which express themselves as distant, smooth ridges (see in photo 1-3)

On basswood - Tilia americana leaf, on petiole, tiny cup fungi.
Apothecia 0.7-0.8mm in length.
Asci 8-spored, IKI+b, 36-46x5-6um, with croziers.
Ascospores eguttulate OCI=0, aseptate, measured
*(7) 7.3 - 8.2 (8.6) × (1.7) 1.8 - 2.2 (2.4) µm
Q = (3) 3.5 - 4.2 (4.8) ; N = 16
Me = 7.7 × 2 µm ; Qe = 3.8
Paraphyses cylindrical with long VBs.

MRD0015

Station : Forêt récréative Dudemaine

Habitat: Forêt mixte (épinette blanche, sapin baumier, pin gris, peuplier faux-tremble

Substrat : Au sol, sur la litière du sol de feuillus

Espèce inconnu

Collectionneur : Mathias Rocheleau-Duplain
Déterminateur :

Long necked Coelomycetes on a relatively young, recently fallen dead Acer rubrum.

Neck about 1mm tall at most, brownish-orange, progressively paler to hyaline at the tip. Conidia produced in a partially immersed pycnidia, conidiogesis apparently annelidic or possibly phiallidic, but clearly entheroblastic.

Conidia cylindrical, sometime very slightly clavate, hyaline, smooth and aseptate measuring 18-21 - 6-7.5(8) avg 19.3 - 7.

based on conidiogenesis and conidia, I am tempted to identify this as a member of the genus Cryptosporiopsis, but I am unable to get to an exact species in verkley monograph, C. coryli is probably close, but differ in its host. However, since I am not familiar with Coelomycetes, I might be completly wrong

growing out of what I believe to be a blueberry (possibly V. cestpitosum). Final image shows comparison with one that I think was growing out of a cranberry (V. oxycoccos - the lower one)

I’m really at a loss on this one. It’s odd that the sphagnum is white. There was some whitish mycelium or something on the sphagnum, but the orange parts aren’t elevated as illustrated for Mitrula. Also, this was cup-shaped, unlike the example Mitrula. Any thoughts?